The invention relates to the technical field of medical treatment devices and particularly relates to a kit for determining the thyroxine content. The kit comprises a box body and a partition board, wherein the partition board is horizontally mounted in the box body to divide the inside of the box body into an upper box body and a lower box body, a support plate is horizontally and fixedly connected in the upper box body, a plurality of reagent pipe holes are formed in the support plate in an equal interval manner and are arrayed in a rectangular manner, a plurality of mutually parallel connecting plates are horizontally arranged on the upper surface of the support plate, the adjacent connecting plates are mutually hinged, the outermost connecting plate is hinged to the side surface of thesupport plate, and second reagent pipe holes corresponding to the first reagent pipe holes are formed in the connecting plates; and a lifting device is horizontally arranged below the support plate.The kit is simple in structure, low in production cost and convenient to use, has a relatively good adjusting function and can be applicable to the placement of different sizes of test tubes.