The invention discloses an in-vivo detection electrode correction method and an application thereof. The method comprises the steps as follows: before in-vivo analysis, an electrode is immersed in anartificial marrowbrain solution containing bovine serum albumin, a to-be-detected substance is added to the artificial marrowbrain solution containing bovine serum albumin, a correction curve of the to-be-detected substance is acquired, and thus, the electrode is corrected. Therefore, the requirement for operation accuracy in the correction process can be reduced, the condition that data obtainedthrough in-vivo analysis cannot be used due to electrode damage after in-vivo analysis is avoided, and the accuracy of detection results of in-vivo analysis is improved.