The invention discloses a train compartment damping rubber mat used between a train compartment and a chassis. The train compartment damping rubber mat comprises an upper plate cooperating with the compartment, a lower plate cooperating with the chassis and a damping layer arranged between the upper plate and the lower plate. The invention further discloses a preparation method of the train compartment damping rubber mat. The train compartment damping rubber mat comprises, by mass, 100 parts of natural rubber, 4 parts of nano-zinc oxide, 1.5 parts of stearic acid, 0.5 part of a scorch retarderCTP, 35 parts of high-wear-resistance carbon black, 3.4 parts of powdered sulfur, 2.5 parts of an accelerant CZ, 1.8 parts of an accelerant DM, 50 parts of light calcium carbonate, 20 parts of oil shale ash and 2 parts of beewax. The preparation method comprises the processes of feeding, thin-passing plastifying, mixing, discharging and sulfuration. The train compartment damping rubber mat is simple in structure, low in cost, high in bearing capacity and good in buffer performance. The rubber part of the damping layer has good breaking strength and elongation at break, high hardness and the low compression set quantity, the rubber mechanical property is excellent, and the train compartment damping rubber mat is suitable for damping of large weight. The train compartment damping rubber matfurther has the advantages of energy saving and environment protection.