The invention relates to a calibration method of electronic-tag sensitivity testing. The calibration method is realized through an RFID-tag sensitivity testing system. A transmitting antenna, a receiving antenna and a tested tag are placed in a dark room or an open field, and a specific distance between the transmitting antenna and the tested tag is measured; actual starting power at the time whenthe tag is activated is checked according to transmitting power; when the tested tag is activated, a corresponding tested-tag query command Query is sent, the tested tag responds with a correspondingresponse signal, and the system carries out CRC calibration verification on the response signal of the tag; if calibration verification is successful, it is shown that a current communication processis successful; and otherwise, a transmitting end continuously sends the query command until the communication process is successfully completed. The method has the advantage of obtaining high-accuracy high-precision electronic-tag sensitivity through using the size of power, which is received by the standard antenna with known gain, as a calibration parameter of sensitivity testing of the electronic tag.