The invention belongs to the technical field of Acer pentaphyllum cultivation, and discloses a cultivation method for rapid propagation of Acer pentaphyllum twigs. Budding twigs are adopted to breed Acer pentaphyllum seedlings, the budding twigs have a plurality of budding stem segments, so that materials are easy to obtained; after the budding twigs are sterilized, germination of clustered shootscan be induced, less materials are needed, and inducements are not limited by small number of amount and seeds of existing Acer pentaphyllum theoretically so that germination amount and germination efficiency of the clustered shoots and axillary buds of the budding stem segments can reach the maximum in a short time. According to the cultivation method, under the premise of ensuring maximum proliferation efficiency, continuous germination of the clustered shoots and the axillary buds of the budding stem segments in the process of proliferative cultivation is promoted to improve survival rateand yield of the Acer pentaphyllum seedlings; adding new ingredients in a strong seedling medium is not needed, so that proportioning costs of the medium are greatly reduced, and by adopting the cultivation method to conduct seedling hardening, seedling-hardening survival rate of the Acer pentaphyllum seedlings can reach more than 80%.