The invention provides PVC (polyvinyl chloride)-based composition, PVC gloves and a preparation method of the PVC gloves. The provided PVC-based composition is prepared from PVC paste resin, a plasticizer, a stabilizer and a viscosity reducer, wherein the plasticizer is prepared from acetyl tributyl citrate and epoxidized soybean oil; the viscosity reducer is 2,2,4-trimethyl-1,3-pentanediol-diisobutyrate. Compared with conventional PVC gloves, the PVC gloves are prepared with a specific formula, VOC (volatile organic compound) emission can be greatly reduced, the atmospheric contamination is reduced, the PVC gloves are safer and more environmentally friendly and do not harm human bodies, and besides, equivalent mechanical performance can be guaranteed. The provided preparation method is simple and easy to implement, mild in condition, easy to operate, environmentally friendly and favorable for large-scale production and meets the environmental protection and green development concept.