The invention relates to a method for preparing Pickering emulsion stabilized on the basis of double-sided particles and application of the Pickering emulsion to immobilized enzymes. The method includes that amphiphilic SiO2 nanometer particles are used as stabilizers, n-heptane is used as oil phase, PBS (phosphate buffer solution) is used as water phase, mixed liquid is stirred by a high-speed homogenizer to form the uniform white Pickering emulsion comprising amphiphilic SiO2 particles. The method and the application have the advantages that the amphiphilic SiO2 nanometer particles are mainly prepared by the aid of paraffin protective modification processes, then the single-wall stabilized Pickering emulsion is assembled on oil-water interfaces, enzymes can be immobilized in water phasesof emulsion, and accordingly the stability and the application performance of the Pickering emulsion can be measured; diversified enzymes can be coated by the Pickering emulsion, accordingly, enzymemolecules can be in sufficient contact with reaction substrates, and the stability and the reusability of the immobilized enzymes can be improved; the novel Pickering emulsion prepared by the aid of the method is low in raw material cost and excellent in economic feasibility and practicality.