The invention relates to the technical field of plant tissue culture, and provides a Phyllanthus emblica tissue culture method which includes the steps: taking semi-lignified branches of Phyllanthus emblicas as explants; sequentially performing induction culture, multiplication culture and rooting culture; performing acclimatization and transplant on acquired rooting seedlings to obtain tissue culture seedlings of the Phyllanthus emblica. A rooting medium of the Phyllanthus emblicas takes 1/2MS medium as a standard, and comprises 0.2-0.8mg/L NAA, 0.1-0.6mg/L KT, 0.1-0.2mM cerium-containing soluble compounds, 8-11g/L agar and 25-35g/L cane sugar. The rooting medium can facilitate forming and growing of bud seedling roots, rooting rate can reach 80-85%, and the method effectively solves thelimit of low rooting rate in the Phyllanthus emblica tissue culture breeding process in production.