The invention belongs to the technical field of biology, more specifically relates to a protein determination kit, and concretely relates to a haptoglobin determination kit. The haptoglobin determination kit comprises a reagent 1 and a reagent 2 which are independently packaged. The reagent 1 comprises 50-150 mM trismetyl aminomethane buffer, 3-12 g/L of polyethylene glycol monooleyl ether-35, 3-8g/L of bovine serum albumin, 4-8 g/L of sodium chloride, and 0.1-0.3 mL/L of a liquid biological antiseptic. The reagent 1 and the reagent 2 comprises a reinforcing agent, and after the reinforcing agent is added, the stability of the reagent is greatly increased.