The invention discloses a personal electric shock prevention control method of a power distribution network with a neutral point which is not connected with ground. The personal electric shock prevention control method comprises the steps of firstly, acquiring a three-phase voltage instantaneous value on a 10kV outgoing line at a substation; secondly, extracting a zero-sequence voltage by the three-phase voltage instantaneous value, and calculating three-phase voltage abruptly-changing quantity; thirdly, judging whether single-phase human body electric shock accident is generated or not according to an integral criterion combined by a zero-sequence voltage absolute value and the three-phase voltage abruptly-changing quantity, and controlling a circuit breaker to be closed; and finally, judging accident removal by the zero-sequence voltage, and controlling the circuit breaker to be disconnected. According to the method, single-end three-phase voltage information is employed, personal electric shock prevention rapid control of the power distribution network with the neutral point which is not connected with the ground is achieved, and the personal electric shock prevention control method has important significance to personal safety protection and normal power supply.