The invention discloses a di-caffeoyl succinyl methyl ester quininic acid compound as well as a preparation method and an application. The compound is a compound 1 or a compound 2, wherein the chemical structure of the compound 1 is as shown in the specification; and the chemical structure of the compound 2 is as shown in the specification. Through separation study on a burdock extract, two novelcompounds are discovered for the first time, and the two compounds have remarkable antidiabetic activity.