The invention relates to the field of lubricating agents and discloses a compound with thickening effect, lubricating grease and preparation methods of the compound and the lubricating grease. The compound is shown in formula I. The preparation method of the compound comprises steps as follows: zircon salt of hydroxyl fatty acid, polyisocyanate, amine and optional another isocyanate react, and thecompound with one or more -NHCONH- structures is obtained. The lubricating grease contains at least one compound. The preparation method of the lubricating grease comprises the steps as follows: zircon salt of hydroxyl fatty acid, polyisocyanate, amine and optional another isocyanate react, and a compound containing a group with one or more -NHCONH- structures is obtained; the product is refinedand the refined product is mixed with the rest base oil and optical additives. The invention provides the lubricating grease prepared with the method. The compound has excellent comprehensive performance and properties of the lubricating grease can be improved.