The invention discloses a plant tissue trauma needle. The plant tissue trauma needle comprises a reverse connecting rod, a needle disk, puncturing needles, cutting blades, a scalding cake and an anti-skid sleeve; the reverse connecting rod is a cylinder; the center of the needle disk is connected with one end of the reverse connecting rod; nine puncturing needles with consistent thickness and length are distributed on the needle disk uniformly; the other end of the reverse connecting rod is connected with the center of the scalding cake; the cutting blades are symmetrically fixed on the two sides of the reverse connecting rod beside the scalding cake; and the anti-skid sleeve engraved with stripes is fixed on the middle part of the reverse connecting rod. According to the plant tissue trauma needle, the problems in the past plant pathogeny trauma inoculation method that the operation of the trauma technology is large in required quantity of test devices, a large amount of devices are inconvenient to carry and keep, and the puncturing and scalding area cannot be fixed are solved, the test devices are convenient to carry and keep, and one device can meet various requirements and canfix the puncturing or scalding area.