The invention discloses a serum replacement for cell culture. The serum replacement is prepared from the following raw materials: manganese chloride, sodium selenite, ammonium molybdate, ferric citrate, choline bitartrate, vitamin D2, vitamin K2, vitamin K3, adenine, guanine hydrochloride, uracil, linoleic acid, cholesterol, insulin, hydrocortisone, dexamethasone, yeast hydrolysate, lactoprotein hydrolysate, casein hydrolysate and malt peptone. The serum replacement disclosed by the invention is added into a common basic culture medium and can be used for culturing cells, and can completely replace serum; the serum replacement does not contain IgG so that a monoclonal antibody produced antibody purification technology is greatly simplified; the cell culture is remarkably prolonged and thecells do not die; the production of protein and antibodies is facilitated; the cost can be greatly reduced and the production cost can be reduced.