The invention discloses a separation guide conveying mechanism suitable for short cigarette production. The conveying mechanism comprises a cutting drum wheel, a conveying drum wheel and a separatingdrum wheel which are arranged parallel to one another; a flow guide plate is movably arranged on the separating drum wheel; a fixing part is arranged on the convex surface of the outer side of the flow guide plate and is connected to a connecting part through a pin; one end of the connecting part is fixed to a valve seat of the separating drum wheel through a fastener; the other end of the connecting part is provided with a fixed handle; and a guiding plate matched with the flow guide plate is arranged on the concave surface of the inner side of the flow guide plate in the radial direction. The conveying mechanism has the advantages that high-precision and high-reliability separation can be realized, high-precision and high-reliability guiding in the cigarette separation process can be realized, the requirement of the complex load environment for cigarette separation can be met, meanwhile, the control over the cigarette separation track can be realized through the mutual tangential andnormal acting force, the conveying mechanism can adapt to separation of cigarettes with different functions, different shapes and different volumes, and convenience can be provided for twisting forming of the cigarettes in the next working procedure.