The invention discloses a multi-layer prefabricated steel concrete shearing wall structure and a preparation method thereof and a construction method thereof. Mounting holes are formed in outer side flanges of end cross beams at the upper end or the lower end of the multi-layer prefabricated steel concrete shearing wall structure; connecting steel plate seats are arranged at the end, not providedwith the cross beams, of the multi-layer prefabricated steel concrete shearing wall structure, and mounting holes are also formed in the connecting steel plate seats to correspond to the mounting holes in the flanges of the outer sides of the end cross beams in position; and technological grooves for mounting bolts or nuts are formed in parts, corresponding to the mounting holes in the outer sideflanges of the end cross beams in position, of prefabricated concrete. According to the multi-layer prefabricated steel concrete shearing wall structure, the factory prefabricated extent is high, andon-site wet operation is little; and fabricated steel concrete shearing walls as well as the fabricated steel concrete shearing walls and peripheral steel beams are in bolted connection, the on-site construction speed is high, the anti-seismic performance is good, the multi-layer prefabricated steel concrete shearing wall structure can be suitable for high-seismic-intensity areas, and the structural applicable height is large.