The invention provides a lycium ruthenicumtissue culture adventitious bud ex-vitro rooting method, which belongs to the technical field of lycium ruthenicum tissue culture. The method comprises the following steps of (1) culturing lycium ruthenicum stem sections into an adventitious bud culture medium to obtain an adventitious bud; (2) directly hardening the adventitious bud obtained in the adventitious bud culture medium to obtain an adventitious bud with a root primordium; feeding a rooting powder solution into the adventitious bud culture medium during a seedling hardening process; (3) sterilizing the adventitious bud with the root primordium, soaking a root part through an IBA solution, transplanting into a substrate, and culturing to obtain a lycium ruthenicum grown-up seedling. The method is simple and convenient to operate and low in culture cost; the whole production cost is reduced by not less than 20 percent; the method is more suitable for mass industrialized production andvariety breeding of lycium ruthenicum seedlings, lays a solid foundation for building a lycium ruthenicum asexual rapid propagation system and providing seedlings for ecological environment construction in northwest China, and has better economic benefit, social benefit and ecological benefit.