The invention belongs to the technical field of laboratory equipment cleaning and particularly relates to a device capable of cleaning sample introduction bottles automatically. The automatic cleaningdevice for the glass sample bottles aims at overcoming defects of the prior art and achieving the effects that interactive cleaning is conducted in a manner that different types of solvent can be adopted based on different pollutants in the sample bottles, and the automatic cleaning device is high in efficiency and portable. According to the technical scheme, the automatic cleaning device for theglass sample bottles comprises solvent bottles, plunger pumps, a pipeline, support feet, solvent spray heads, the sample bottles, a sample bottle wheel disc, a seal shaft, a speed reduction motor, abase plate, a glass housing, a nozzle base and a seal plug; the base plate is a disc, wherein a through hole is formed in the center of the disc; the glass housing is buckled onto the base plate; theseal plug is arranged between the lower edge of the glass housing and the base plate; the speed reduction motor is arranged below the base plate; the seal shaft is arranged on the speed reduction motor, penetrates the central through hole of the base plate and extends into the glass housing; and the sampled bottle wheel disc is arranged at the upper end, inside the glass housing, of the seal shaft.