At present, when tobacco seedlings are transplanted by a tobacco grower, in general, seedling culture trays are stacked in a soil basket layer by layer to be carried to a tobacco field where tobacco seedlings are to be transplanted, however, the tobacco seedlings are prone to be pressed and damaged when placed in the manner, and unnecessary loss can be caused. The invention aims to provide a layered anti-pressing tobacco carrying frame. The carrying frame is composed of a plurality of layers of tobacco placing frames, the seedling culture trays are placed on the tobacco placing frame, each layer of the tobacco placing frame is provided with one tray of the tobacco seedlings, then root systems and stems of the tobacco seedlings cannot be damaged at all, so that the survival rate of the tobacco seedlings is improved, and the transportation efficiency of the tobacco seedlings is also improved. The carrying frame is mainly composed of the tobacco placing frame, hollow pipes and rope tyingholes, and is characterized in that the multiple layers of the tobacco placing frames are combined together through the hollow pipes, each layer of the tobacco placing frame is a tray frame composed of four long rods and two short rods, the four corners of each tray frame are each provided with a straight rod, the diameter of each straight rod is slightly smaller than the inner diameter of each hollow pipe, the straight rods just can be inserted into the hollow pipes, and one rope tying hole is formed in each of the four corner edges of the bottommost layer of the tobacco placing frame.