利索-全球专利检索

  1. 登录
  2. 注册
发明授权
CN108017694B pORF65重组蛋白及其制备方法和应用 有权 许可

pORF65重组蛋白及其制备方法和应用
摘要：
本发明公开了pORF65重组蛋白及其制备方法和应用，所述pORF65重组蛋白依次由SEQ ID NO.17—SEQ ID NO.21所示氨基酸序列通过连接子连接而成，所述连接子为4－7个分子量小的、极性且亲水的氨基酸构成。经ELISA显示，本发明所述pORF65重组蛋白可以作为包被抗原，能很好地用以检测CyHV‑3 pORF65完整编码序列构建的DNA疫苗免疫锦鲤后产生的血清特异性抗体，以及诊断锦鲤是否感染鲤疱疹病毒3型。
摘要（英）：

The invention discloses a pORF65 recombinant protein and a preparation method and application thereof. The pORF65 recombinant protein comprises amino acid sequences shown in the formulas of SEQ ID NO.17 to SEQ ID NO.21 orderly connected by connexins, and the connexin is composed of 4-7 hydrophilic amino acids with small molecular weight and polarity. The ELISA result shows that the pORF65 recombinant protein can be used as a coating antigen and can well detect a serum-specific antibody produced by a cryprinus carpiod immunized by a DNA vaccine constructed from a complete coding sequence of CyHV-3 pORF65 and diagnose whether the cryprinus carpiod infects cyprinid herpesvirus III.

公开/授权文献
0/0