The invention provides a two-section independent temperature-control biological 3D printing spray nozzle. The two-section independent temperature-control biological 3D printing spray nozzle comprisesa main shell, a material barrel, a spraying nozzle extruding device and a spraying nozzle needle, a first temperature-control device is arranged in the main shell, the material barrel is inserted intothe first temperature-control device, and the bottom end of the material barrel is connected with the spraying nozzle extruding device through a feeding pipe. A discharging pipe is arranged at the bottom end of the spraying nozzle extruding device, the spraying nozzle needle is connected with the discharging pipe and penetrates out to the outer side of the bottom of the main shell, a second temperature-control device is arranged between the tail end of the spraying nozzle needle and the top end of the discharging pipe, and a heat isolating device for preventing mutual interference of the temperatures is arranged between the first temperature-control device and the second temperature-control device. According to the two-section independent temperature-control biological 3D printing spray nozzle, independent temperature control over the spraying nozzle material barrel section and the spraying nozzle needle section is achieved, gradient temperature control of the spraying nozzle can be achieved, all gradient values are mutually independent, and temperature control is not affected by one another.