A dust mite attractant screening device and a method thereof are disclosed. The device includes a trap, a storage pot and a separator. The storage pot contains dust mites, and is connected to the trapthrough connecting strips. An attractant to be tested is put on the trap, the dust mites in the storage pot enter the trap through the connecting strips and eat the attractant under functions of theattractant to be tested, the trap is disposed in the separator so that the dust mites in the trap can be separated from the attractant to be tested, and the attracting effect of the attractant to be tested can be determined through weighing the residual amount of the attractant to be tested. The attracting effect of each attractant to be tested is evaluated through the device and the screening method based on the device, effective components of the attractant are determined according to the attracting effects, and effective attractants are found to form a formula. The device and the method areaimed at determination of an efficient, nontoxic and pollution-free dust mite attractant formula by means of technical research, and have a wide application prospect.