The invention belongs to the gold hydrometallurgy operation status field and provides a gold hydrometallurgy full-flow layer and block operation status on-line evaluation method. The method includes three sub-processes, namely, primary soaking and primary washing, secondary soaking and secondary washing, and zinc powder replacement. Layer and block evaluation models are established for the full flow of gold hydrometallurgy according to the differences of operation units, and are divided into a full-flow layer, a sub-process layer and a unit layer. The algorithm includes the establishment of the layer and block evaluation models, the online evaluation of process operation statuses, and the traceability of reasons for poor operation statuses. According to the method of the invention, when online evaluation is performed, similarities between online data and the evaluation models are calculated, so that the operation status of the full flow of the gold hydrometallurgy is evaluated in realtime, and therefore, the problem of the lagging of manual evaluation is avoided; reasons for poor operation are automatically traced, so that valuable guidance is provided for an actual production process, and therefore, the operation status of the process can be timely adjusted, and the economic efficiency and production efficiency of enterprises can be improved.