The invention discloses an antibacterial tatami. The antibacterial tatami includes a mat body, and the mat body comprises a fabric layer on the upper surface, a middle core material, and an antislip cloth on the lower surface. The fabric layer is woven from a warp yarn and weft yarn, and the weft yarn is added with an antibacterial agent, which includes, by weight, 70-85 parts of silver-loaded zeolite, 1-2 parts of silver-loaded zirconium phosphate, 5-8 parts of isothiazolinone, 2-5 parts of guazatine acetate, 1-5 parts of a titanate coupling agent, 1-5 parts of polyurethane resin, and 2-3 parts of a pyrithione salt. Through modification of the warp yarn and weft yarn on the fabric layer respectively, the fabric layer of the obtained antibacterial tatami has certain antibacterial properties, and at the same time the latex mat adopts natural latex and artificial latex as the main raw materials, and has high elasticity.