The invention discloses a preparation method for biological glue for fracturing. The method is realized through the following steps: step 1: preparing a water phase from the following components in parts by weight: 40 to 88 parts of water, 2 to 10 parts of rosin-based polyoxyethylene sodium sulfosuccinate monoester and 10 to 50 parts of acrylamide; step 2: preparing an oil phase from the followingcomponents in parts by weight: 90 to 99 parts of white oil and 1 to 10 parts of Span 80; step 3: preparing emulsion from the following components in parts by weight: 30 to 60 parts of the water phaseprepared by the step 1 and 40 to 70 parts of the oil phase prepared by the step 2; under a stirring state, slowly adding the water phase into the oil phase; after adding the water phase, performing circular emulsifying for 1 to 3 times by utilizing a homogenization emulsifying pump; step 4: carrying out a polymerization reaction: adding the emulsion prepared by the step 3 into a reaction kettle with a stirrer; performing stirring and introducing nitrogen gas for 10 minutes or more; adding 0.005 to 0.1 part of an oxidant and raising the temperature to be 35 to 45 DEG C; then slowly dropwise adding 0.005 to 0.1 part of a reducing agent and completing the adding after 2 to 5 hours; and after completing the adding of the reducing agent, preserving heat at 35 to 45 DEG C for 1 hour, so as to prepare the biological glue.