The invention discloses a clotting time determination method, a clotting time determination device and a clotting time determination system. In the clotting time determination method, a steel ball amplitude value indicated by a currently acquired signal value is always compared with a steel ball amplitude value indicated by a reference signal value, and the steel ball amplitude value indicated bythe currently acquired signal value is always compared with a steel ball amplitude value indicated by a preset signal value; if the steel ball amplitude value indicated by the currently acquired signal value is greater than the steel ball amplitude value indicated by the reference signal value, then the currently acquired signal value is used for updating the reference signal value; and if the steel ball amplitude value indicated by the currently acquired signal value is less than the steel ball amplitude value indicated by the preset signal value, clotting detection timing is stopped. The clotting time determination method can effectively solve the problem that a determination result of clotting determination is inaccurate.