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发明公开
CN108060133A 稳定低表达miR-497的HPMC细胞株及其应用 有权

稳定低表达miR-497的HPMC细胞株及其应用
摘要：
本发明公开了一种稳定低表达miR‑497的HPMC细胞株及其应用。稳定低表达miR‑497的HPMC细胞株的名称为：人腹膜间皮细胞稳定表达下调miR‑497，保藏编号为：CCTCC NO：C2017235，其基因组中含miRNA‑497inhibit质粒。用嘌呤霉素筛选HPMC稳转细胞株的筛选浓度为1.5～2.5μg/ml。HPMC稳转株中miR‑497的表达与VEGF呈负相关。本发明制备出的稳定miR‑497表达下调的HMPC细胞株可用于进一步的动物实验甚至临床试验，可用于探索PF的发生机制，寻找PF新的缓解措施，为患者继续维持PD开辟新道路。
摘要（英）：

The invention discloses an HPMC strain stably underexpressing miR-497 and application thereof. The HPMC strain stably underexpressing the miR-497 is named as the human peritoneal mesothelial cell strain stably reducing expression of the miR-497, the collection number is CCTCC NO: C2017235, and a miRNA-497inhibit plasmid is contained in a genome thereof. The screening concentration for screening the HPMC stable expression strain by using puromycin is 1.5-2.5[mu]g/ml. In the HPMC stable expression strain, expression of the miR-497 is negatively correlated with VEGF. The HPMC strain stably reducing the expression of the miR-497, provided by the invention, can be applied to further animal experiments or even clinical trials, and can be used for exploring the occurrence mechanism of PF so as tofind new mitigating measures for PF and open up a new path for patients to continue maintaining PD.

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