The invention discloses a DNA typing identification kit which comprises a group of primers used for multiple PCR (Polymerase Chain Reaction), and the primers have sequences shown as SEQ ID No.1-52. The DNA typing identification kit disclosed by the invention is capable of simultaneously amplifying 22 genetic loci in the same reaction tube and simultaneously detecting gender loci and 21 euchromosome loci. DNA fragments of the 22 genetic loci are simultaneously amplified in the same reaction tube, so that the diagnostic efficiency and diagnostic accuracy can be greatly improved, the cost is reduced, and the detection time is shortened.