The invention provides a PCB supporting handshaking device and a PCB handshaking method. The PCB supporting handshaking device comprises supporting handshaking blocks, connecting plates, cylinders andvacuumizing devices; the supporting handshaking blocks are fixedly connected with the connecting plates, adsorption grooves are formed in the supporting faces of the supporting handshaking blocks, the vacuumizing devices are communicated with the adsorption grooves, and the connecting plates are connected with the cylinders; when PCBs are subjected to handshaking, the adsorption grooves are vacuumized through the vacuumizing devices, the PCBs are adsorbed through the adsorption grooves accordingly, can be prevented from being jacked over or sliding down, the fluency of PCB handshaking is improved, and the fragmenting risk of the PCBs is reduced.