The invention discloses a plastic foam insulation box with an interlayer. The plastic foam insulation box comprises a box cover and a box body, wherein a raised line is arranged at the bottom of the box cover, a first groove is formed in the box cover, two opposite partitions are arranged in the middle of the first groove, a limiting hole is formed in the bottom of the groove, the raised line is clamped and connected with the top of the box body through a clamping groove, a clamping groove angle bead is arranged at the corner in the clamping groove, a first angle bead is arranged in an openingof the box body, four foam columns are arranged at the corners in the box body, a second groove is formed in the inner bottom end of the box body, and the interlayer is arranged in the box wall of the box body. According to the plastic foam insulation box, the box body is designed into a cavity, so that the use space of the insulation box is large, the space of the box body can be preferably andreasonably used by people during use, and the self structure of loaded goods is not limited, so that the loading capacity of the box body is greatly increased.