The invention provides a printing ink cleaning agent and a preparation method thereof. The printing ink cleaning agent comprises the following components in parts by weight: 10-15 parts of isopropyl myristate, 4-7 parts of oleic acid, 5-10 parts of polysorbate, 1-5 parts of polyoxyethylene ether, 4-8 parts of butyl methacrylate, 1-5 parts of a silane coupling agent, 1-5 parts of an antioxidant, 1-5 parts of tea saponin, 5-15 parts of ethanol and 80-120 parts of deionized water. The printing ink cleaning agent provided by the invention has high cleaning efficiency, good cleaning effect, small solvent volatility, no-softening substrate, little damage to printing equipment, low toxicity, and little harm to human bodies and the environment, the cleaning of printing machinery is more convenient, and the pollution of the cleaning agent to the environment is reduced.