The invention belongs to the technical field of propagation of herbaceous plants, and discloses a method for tissue culture and rapid propagation of marsh marigold blades in Beichuan. An optimum aseptic culture condition for rapid blades propagation is explored through disinfection conditions, shoot induction and proliferation, rooting and transplanting experiments; 70 % ethanol 1min plus 0.1% litre mercury conduct disinfection treatment on the blades for 30s, a disinfection effect is the best, explants contamination rate is only 5%, survival rate is up to 80%; when using 2,4-D and 6-BA, a best induction effect of adventitious buds is found in MS plus 2mg L<-1> 6-BA plus 1mg L<-1> 2,4-D, the average number of buds reaches 1.67; tissue culture seedlings are conducted rooting culture on a medium 1/2MS plus 0.1mg L<-1>NAA, rooting rate can reach 45%, and the rooting number can reach 2 to 3; in a screening experiment of a transplanting medium, the growth effect of the tissue culture seedlings of the marsh marigold in Beichuan under the conditions of humus to vermiculite equal to 1 to 1 is the best, and the survival rate is up to 48.7%.