The invention discloses a nano-fog disinfection and sterilization device. The device comprises a disinfection shell, the disinfection shell is a three-dimensional cabinet structure, a liquid storage module, an atomization module and a spray module are arranged in the disinfection shell, the atomization module is arranged below the liquid storage module, the spray module is provided with a circulation tube, a miniature air pump is arranged in the circulation tube, the spray module is arranged between the liquid storage module and the atomization module, a circuit control module and a detectionmodule are arranged in the disinfection shell, the circuit control module is connected with the atomization module and the spray module, the detection module is connected with the liquid storage module, and the disinfection shell is provided with a hollow hole. The nano-fog disinfection and sterilization device has a simple structure, can nano-atomize a disinfecting liquid to increase the contactarea with microbial bacteria in the air, so the disinfection and sterilization effect is obvious, and the sterilization efficiency is improved; and a circulation system is additionally arranged to make the disinfecting liquid in air cyclically recovered after being disinfected and sterilized, so harms to human bodies are avoided, and the device has no side effects on articles in a disinfection space, and is worth promoting. .