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发明公开
CN108144121A 一种生物源性小口径组织工程血管的制备方法 有权 转让

一种生物源性小口径组织工程血管的制备方法
摘要：
本发明涉及一种生物源性小口径组织工程血管的制备方法，属于组织工程和生物材料领域。一种生物源性小口径组织工程血管的制备方法，包括如下步骤：(1)冻融：将新鲜动物血管放在含有0.02％～0.2％EDTA的PBS缓冲液中，置于-80℃速冻1～3小时，取出后立刻放置于37℃条件下10～30分钟解冻；重复上述操作2～5次，得到血管材料；(2)去除冻融后脱落的细胞碎片；(3)脱细胞；(4)清除去垢剂。本发明的优点是提供了一种能够脱除血管组织内的细胞成分，并最大程度保护脱细胞基质的结构完整性的脱细胞方法和试剂组合。
摘要（英）：

The invention relates to a preparation method of biogenic small-aperture tissue engineering blood vessels, and belongs to the fields of tissue engineering and biological materials. The preparation method of the biogenic small-aperture tissue engineering blood vessel comprises the following steps: (1) freeze thawing: placing fresh animal blood vessels into a PBS (Phosphate Buffer Solution) containing 0.02 to 0.2 percent of EDTA (Ethylene Diamine Tetraacetic Acid), and performing quick freezing at the temperature of 80 DEG C below zero for 1 to 3 hours, taking the fresh animal blood vessels out,and immediately performing unfreezing at the temperature of 37 DEG C for 10 to 30 minutes; repeating the abovementioned operations for 2 to 5 times to obtain a blood vessel material; (2) removing cell debris fallen after the freeze thawing; (3) removing cells; (4) removing a detergent. The invention has the advantage that a cell removing method and a reagent composition which can effectively remove cell components in blood vessel tissues and protect the structural integrity of acellular matrixes to the maximum extent are provided.

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