The invention relates to an environment-friendly isolated self-rinsing beehive, comprising a base, water tank grooves, and magnetic strips, wherein a box body is located on the base, slot bodies are arranged inside the box body, LED lights are arranged on the right and left sides of each slot body, an isolation box is arranged inside each slot body, a sprinkler head is connected at the right sideof the inner part of each isolation box, a connecting rod is connected at the top of each isolation box, a slider is fixed on each connecting rod, a fixed block is arranged outside each slider, the water tank grooves are arranged at the right sides of the slot bodies, a spring is connected at the top of each water tank groove, the bottom of each spring is connected to a water tank, a first observation port is arranged at the outer surface of each water tank, and a door body is arranged at the outer surface of each isolation box. The invention has the advantages that the environment-friendly isolated self-rinsing beehive is provided with eight slot bodies, the isolated slot bodies can be used for breeding the bees separately, thereby preventing the situation of interaction between bees.