The invention discloses a molecular marker closely linked to nakedseed traits of Asian cotton DPL972 and application. The molecular marker is located on a 6th chromosome A08, the forward primer sequence of the molecular marker is shown as SEQ ID No.1, and the reverse primer sequence of the molecular marker is shown SEQ ID No.2. A pair of primers for identifying Asian cotton nakedseed materials arefound out. 80-90% of phenotypic variation in an Asian cotton natural population can be explained. The molecular marker can be used for molecular marker-assisted selection of the nakedseed traits andfine mapping and map-based cloning of nakedseed genes, and a certain theoretical basis is provided for cotton fiber initiation research, fiber quality improvement and cotton genetic breeding.