The invention discloses a scouring agent, and a preparation method and application thereof. The scouring agent comprises the following raw materials by weight: 15 to 20% of alkyl diphenyl ether sulfonate, 10-15% of alkyl glycoside, 5-10% of isomeric alcohol polyoxyethylene ether, and 5 to 10% of silicon-modified polyether, with the balance being water, wherein the silicon-modified polyether has amolecular weight of 200 to 2000 g/mol and has a general structural formula as described in the specification. In the formule, R is a straight-chain or branched-chain C6-18 alkyl group; R' is a straight-chain or branched-chain C1-10 alkylene group or a C6-10 arylene group; m is in a range of 1 to 20; n is in a range of 0 to 20; and m and n are both integers. The scouring agent provided by the invention is high efficient and environment friendly; the components of the scouring agent produce synergism; and the scouring agent has the advantages of acid resistance, alkaline resistance, rapid permeability, excellent low foam performance, alkali-resistant stability, high cloud point and high surface activity. A fabric treated by the scouring agent has satisfactory capillary effect and whiteness,and can meet requirements of subsequent dyeing and printing processes and other processes.