The invention relates to a motorcycle axle detection device which comprises a machine frame, the machine frame comprises a workbench and a sliding plate, the sliding plate is slidably connected to theworkbench, a motor is arranged on the sliding plate, a chuck is rotationally connected to the sliding plate, a belt is connected between the chuck and the output shaft of the motor, a detection rod abutting against the axle is arranged above the workbench, a cavity is formed in the detection rod, a first air bag is arranged in the cavity and the cavity is slidingly connected with a detection pen,the first air bag is connected to one end, far away from the axle, of the detection pen, a second air bag communicated with the first air bag is arranged on the outer side of the detection rod, a pushing frame is arranged between the second air bag and the detection rod, the middle of the pushing frame is rotationally connected to the machine frame, a pushing rod used for extruding the second airbag is arranged on the pushing frame, a spring is connected between the pushing frame and the sliding plate, a connecting rod is connected between the pushing frame and the detection rod, and an intermittent pushing mechanism is arranged on the sliding plate. According to the scheme, in the detection process of the axle, marks are made on the protruding parts of the axle.