A design method for a mask plate of an elliptical optical conveyor belt light beam capable of transmitting and taking in and sending out particles is disclosed. Two elliptical ring factors are combined; after interference of two elliptical ring factors is superposed, a phase step is introduced to form a gap for taking in and sending out particles, a mask plate complex amplitude transmittance function can be obtained, and the mask plate of the elliptical optical conveyor belt light beam capable of transmitting and taking in and sending out the particles can be obtained after an angle of the mask plate complex amplitude transmittance function is determined and blazed optical gratings are superposed. Via the mask plate designed in the present invention, the elliptical optical conveyor belt light beam capable of transmitting and taking in and sending out the particles can be generated at a far field of the mask plate. In addition, one end of the conveyor belt is provided with an opening, and each carrying unit can be sequentially moved to the opening via adjustment and control of transmission factors; in an open state, the particles can be taken in and send out via microflow assistingoperation and the like, and stable capture can be formed. Therefore, the design method has very important application prospects in the field of micromanipulation.