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发明公开
CN108179206A RT-PCR检测猕猴桃溃疡病原菌的引物及检测方法 失效 - 权利终止

RT-PCR检测猕猴桃溃疡病原菌的引物及检测方法
摘要：
本发明提供了一种实时荧光RT-PCR检测猕猴桃溃疡病原菌Pseudomonas syringaepv.actinidae的特异性引物，其由正向引物和反向引物组成，所述正向引物为：5＇-CCGCTGTGATACAATTCGGC-3＇，如SEQ ID NO.1所示，所述反向引物为：5＇-CGCACATAAGACAACAGGCG-3＇，如SEQ ID NO.2所示；利用所述特异性引物进行实时荧光RT-PCR检测比进行普通PCR检测的灵敏度至少提高100倍。本发明还提供基于该特异性引物的试剂盒、检测方法和应用。
摘要（英）：

The invention provides a specific primer for detecting pseudomonas syringaepv.actinidae through real-time fluorescent RT-PCR (Reverse Transcription-Polymerase Chain Reaction). The specific primer consists of a forward primer and a reverse primer. The forward primer is: 5'-CCGCTGTGATACAATTCGGC-3', as shown in SEQ ID NO.1, and the reverse primer is: 5'-CGCACATAAGACAACAGGCG-3', as shown in SEQ ID NO.2. The sensitivity of real-time fluorescent RT-PCR detection by the specific primer is at least improved by 100 times compared with that of common PCR detection. The invention further provides a kit based on the specific primer, a detection method and application.

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