The invention discloses application of a reagent expressed by interference long chain RNA PVT1 in preparing a preparation for adjuvant therapy of nasopharyngeal carcinoma. Interference vectors of PVT1are transferred into cell lines CNE2 and 5-8F of the nasopharyngeal carcinoma; after the expression of the PVT1 is inhibited, the proportion of apoptotic cells is obviously increased, which shows that the reagent expressed by the interference long chain RNA PVT1 can be used for the adjuvant therapy of the nasopharyngeal carcinoma and has significant clinical treatment significance.