The invention discloses a trepang bait additive through an active probiotic culture medium containing traditional Chinese medicines. The active probiotic culture medium containing traditional Chinesemedicines is used for fermenting and culturing microorganisms used in a trepang bait, and comprises the following components of wheat bran, corn flour, brown sugar, traditional Chinese medicine components, light calcium carbonate and yeast cream. The traditional Chinese medicine components comprise radix codonopsis, astragalus membranaceus, Chinese wolfberry fruits, bighead atractylodes rhizomes,poria cocos, radix angelicae sinensis, licorice roots, haws, radix et rhizoma rhei, radix scutellariae, radix gentianae and radix rumex patientia. The trepang bait additive can solve the problems thatin the prior art, the number of the microorganisms in the trepang microecology bait additive is small, the activity is low, and the colonization capacity in intestinal tracts is weak, so that the number of the microorganisms in the trepang microecology bait additive can be increased, and the activity of the microorganisms and the colonization capacity in the intestinal tracts are improved.