The invention relates to the application of Mundoserone to inhibition of zebrafish embryo angiogenesis. Zebrafish is put into Mundoserone medicine bath with different concentration, a medicine with specific anti-angiogenesis effect serves as positive control, a solvent serves as negative control, and the Mundoserone has the effect of inhibiting zebrafish embryo angiogenesis through discovery; furthermore, the inhibition rate is related to the medicine concentration, and on this basis, the influence on the related signal channel in the zebrafish embryo angiogenesis process by the Mundoserone isresearched through Real-time PCR, and the mechanism for inhibiting angiogenesis is defined. Therefore, the Mundoserone can be used for preparing related medicines and used for treating diseases taking angiogenesis as pathological characteristics, such as tumor, diabetic retinopathy, hemangioma, psoriasis, rheumatoid arthritis and menstrual disorder.