The invention discloses a preparation method of helianthus tuberosus essence. The preparation method comprises the steps that helianthus tuberosus is smashed and pulped, vacuum filtration is performedand vacuum concentration is performed, the concentrated juice is subjected to a reaction for 1-5 hours at the temperature of 100-180 DEG C after a sugar source and an ammonia source are added, then 3-10 times volume of an organic solvent is added, and the mixture liquid is heated, and subjected to reflux extraction for 1-4 hours at the temperature of 45-80 DEG C; and the extracting solution is subjected to primary and secondary molecular distillation under a certain condition, and the distilled light phase is the helianthus tuberosus essence. The preparation technology is simple, the cost islow, the obtained helianthus tuberosus essence is uniformly sprayed on tobacco shred through a cigarette flavoring procedure, the baked sweet and burnt sweet fragrance of the cigarette can be highlighted, the smoking quality and the sensory comfort degree of the cigarette are improved, and the preparation method is worthy of being applied and popularized in the whole industry.