The invention relates to the technical field of biologics, in particular to a method for detecting cold resistance of mulberry tree varieties. The method comprises the following steps: preparing an antibody through a specific AKR2A protein sequence, and coating a 96-pore polystyrene reaction plate with the arabidopsis thaliana AKR2A antibody. After specific mulberry leaf tissue treatment, an AKR2Aprotein antigen is prepared, the AKR2A protein antigen is put into an anti-body coated reaction plate according to serial numbers, is subjected to immunoreactions with the AKR2A antibody, and is further subjected to a reaction with an second antibody marked by horseradish peroxidase, and comparison analysis shows that the low-temperature stress resistance of a mulberry tree is in a positive correlative relationship with the content of an AKR2A gene or protein, and the cold resistance of a mulberry tree can be indicated by detecting the content of the AKR2A protein in 1-2 leaves at the top endof the mulberry tree. A direct, rapid and reliable mulberry tree cold resistance detection method can be established, and the method can be applied to screening of cold-resistant mulberry tree variety, molecular seed breeding and long-distance and super long-distance seed introduction of mulberry germplasm (varieties), and has great academic and economic values.