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发明公开
CN108277289A 大肠埃希氏菌O157:H7干粉化LAMP快速检测试剂盒 无效 - 撤回

大肠埃希氏菌O157:H7干粉化LAMP快速检测试剂盒
摘要：
本发明公开了大肠埃希氏菌O157:H7干粉化LAMP快速检测试剂盒，该试剂盒由干粉化检测试剂、复溶液、阳性对照干粉、阴性对照干粉、封闭液、显色液和裂解液组成，以上7种试剂分别置于容器中。应用于本试剂盒中的引物对应大肠埃希氏菌O157:H7特异性基因序列的8个区段，6条引物，并且通过加入显色液，阴阳性显色差异明显，具有特异性强、灵敏度高、易于操作、结果判断简单等优点，适合于食品安全和医药卫生领域中的大肠埃希氏菌O157:H7快速检测。另外，本发明为干粉化的检测试剂，便于常温运输，且使用简单、快捷，无需专门仪器，降低成本并拓宽产品应用范围，更适用于现场及偏远地区基层对大肠埃希氏菌O157：H7的快速检测，具有广泛的应用前景。
摘要（英）：

The invention discloses an Escherichia coli O157:H7 dry powder LAMP rapid detection kit. The Escherichia coli O157:H7 dry powder LAMP rapid detection kit is composed of seven reagents including a drypowder detection reagent, a redissolving solution, a positive reference dry powder, a negative reference dry powder, a confining liquid, a color developing solution, and a lysate; the above seven reagents are introduced into a container. A primer adopted in the Escherichia coli O157:H7 dry powder LAMP rapid detection kit is designed to match 8 sections and 6 primers of Escherichia coli O157:H7 specific gene sequence; the color developing solution is added, so that color development difference of the positive reference and the negative reference is more obvious, the specificity and the sensitivity are high, operation is convenient, result determination is simple, the Escherichia coli O157:H7 dry powder LAMP rapid detection kit is suitable for rapid detection of Escherichia coli O157:H7 in the fields of food safety and medicine science. Dry powder is adopted by the Escherichia coli O157:H7 dry powder LAMP rapid detection kit, so that it is convenient for normal temperature transportation, using is simple and rapid, no special equipment is needed, cost is reduced, product application range is widened, the Escherichia coli O157:H7 dry powder LAMP rapid detection kit is suitable for rapid detection on Escherichia coli O157:H7 in remove regions, and is promising in application prospect.

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