The invention discloses a bacteria stick for gastrodia elata cultivation and a preparation method thereof, and relates to the technical field of gastrodia elata cultivation. The bacteria stick adoptsbamboo as a stick core, the outer layer of the bamboo is wrapped by straw, saw dust or/and a plant growth regulator agent, the bamboo is hollowed, and the straw on the outer layer has multiple gaps, so that the honey fungus oxygen throughput is effectively ensured; the straw and the saw dust and rice paste outside the straw can supply sufficient glycogen and nutrient substances to growth of honeyfungus, and hyphae and gastrodia elata seeds can grow. The bacteria stick is made of waste saw dust or branches, the broadleaf trees are protected against immoderate chopping, and forest resources andenvironment are protected; the contradiction between ecologic environment protection and gastrodia elata promotion planting is solved, a new way is provided for gastrodia elata planting, the stick isapplicable to gastrodia elata planting popularization and expansion, and the modernization, industrialization and large-scale planting scale can be formed to meet the requirement.