The invention relates to the technical field of antibacterial mask preparation, and discloses a method for preparing an antibacterial mask filter disc and a mask using the filter disc. The method forpreparing the antibacterial mask filter disc comprises the following steps that (1) opening and mixing are carried out on a spinning fiber, so that the thickness of the spinning fiber is 0.5-1.0 centimeter, the fiber average diameter of the spinning fiber is 300-400 nanometers, the average bore diameter of the fiber is 1.0-2.0 micrometers, and opening is stopped; (2) the spinning fiber after opening in step (1) is placed on a carding machine of which the mesh outlet speed is 2-4 meters per minute and the temperature is 30 DEG C for carding so as to make the fiber monofibrillation, and therebya fiber surface mesh with a certain surface density and uniform distribution is formed. The method for preparing the antibacterial mask filter disc and the mask using the filter disc have the advantages that multi-level preparation process is carried out on a fiber surface layer, not only is the operation simple, but also the comfort degree of the fiber surface layer is greatly improved, antibacterial powders are compressed in a base material non-woven fabric, and drug properties of lonicerae japonicae flos, rhizoma seu radix notopterygii, ramulus cinnamomi and baical skullcap root are used toplay an effective antibacterial role.