The invention discloses a visual and convenient tail vein injection assisting module. The visual and convenient tail vein injection assisting module comprises an experiment table and a mouse cylinderand is characterized in that a plurality of limit parts are fixedly arranged at the lower end of the mouse cylinder, the mouse cylinder is arranged on the upper end face of the experiment table through grooves which are formed in the upper end face of the experiment table and coordinated with the limit parts, one end of the mouse cylinder is provided with an opening, a head sleeve is arranged inside the opening, one end, away from the opening, of the mouse cylinder is provided with a tail plate, the tail plate is provided with a plurality of first screw holes, the center of the tail plate is provided with a first tail hole, the end face, on one side away from the mouse cylinder, of the tail plate is provided with a squeezing module, the squeezing module comprises a first fixing plate and asqueezing air bag, and the first fixing plate is provided with second screw holes coordinated with the first screw holes in position and size. The visual and convenient tail vein injection assistingmodule has the advantages that the mouse vein can be easily observed during mouse tail vein injection to increase injection accuracy, experiment efficiency is increased greatly, and use convenience isachieved.