The invention discloses a method for regulating and controlling the diversity of fungi in airport soil by a waste and old colloidal particle-filled matrix. The method comprises cutting plants in a regulating and controlling zone, turning soil in the zone once at depth of 20cm, applying 100 t/ha of waste colloidal particles for soil matrix filling in the regulating and controlling zone in each 6.7m*5 m of a sample field, uniformly mixing the colloidal particles and the turned soil, wherein the plants in the filled regulating and controlling zone are in a natural growth state, after the regulating and controlling matrix filling, acquiring soil samples in the matrix regulating and controlling zone respectively in the autumn of the same year, the spring of the next year and the summer of thenext year, and testing the soil samples. The method can effectively regulate and control the vegetation community structure on the ground and insect number characteristics, reduce bird food sources orcut off the bird food chain to achieve the purpose of airport ecological bird control, and accumulate the basic ecological information for bird attack prevention and control in the airport based on the ecological method.